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The research was carried out at Univates' Laboratory of Ecology and Evolution, coordinated by Professor Eduardo Périco, who supervises the students in Brazil. The laboratory's master's fellows, Pauline Vognach and Cléber Sganzerla, also participated in all the work, especially in field collections and species identification. In Sweden, research was overseen by researchers Antonia Liess and Göran Sahlen, with whom Périco has research partnerships.

To conduct the research, the students used aquatic insects and related richness (number of species), abundance and composition (which species were present) in three different sampling points: organic rice fields, conventional rice fields (with pesticides and fertilizers) and natural wetlands (control areas, with no cultivation). The objective was to verify whether the aquatic insect community varied between areas and, based on the results, to be able to use these organisms as indicators of the degree of anthropization of the environment.

The results did not show a significant influence of nitrogen on the richness or abundance of insects and nitrite shows a significant influence, but not enough to make significant inferences. The water parameters with the highest correlation with the insect community in this study were pH, redox potential (ORP), conductivity and total dissolved solids. These results indicated that in conventional cultivation (with the use of pesticides and fertilizers) the water became more acidic and with greater conductivity, favoring more generalist insect species, changing the composition of the insect community in relation to areas with organic cultivation and without cultivation ( natural areas).

Both the measured pH value and the ORP are strongly correlated with the chemical state of the water and the insects that live there. A pH value that is too high or too low is not favorable for aquatic insects or water biodiversity, nor is an ORP value that is too low.

The students argue that the focus of agricultural practices must ensure that the basic natural ecological processes remain functional in all Brazilian biomes, such as the Pampa biome, where collections are collected. An alliance between sustainable development and the implementation of food security is needed. Therefore, research is needed in several areas, such as agricultural systems, which may be useful for future generations and environmental well-being, such as conservation and sustainable use of biodiversity.

Tekla thus explains the results. “The main results of the research showed that the biodiversity of aquatic insects differed between locations in relation to water quality. But the results could not prove any significant difference in the composition of aquatic insects in the three growing areas analysed.”

The young woman says that she hopes to be able to continue studying the zones known as wetlands in the future. “In general, sympathy for nature is an essential component of ecological thinking. Human change in land and water use must be an integral part of future agricultural practices, as well as human behavior towards nature in general, both socioeconomic and biological,” she says.