Expressão do gene ESR1 em tumores de mama canino
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Data
2023-12-19
Autores
Orientador
Bustamante Filho, Ivan Cunha
Banca
Fernandes, Cristina Gewehr
Majolo, Fernanda
Luz, Marcelo Rezende
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Resumo
O câncer é a causa mais comum de morte em cães em todo o mundo, sendo que os tumores mamários constituem a neoplasia mais frequente em fêmeas caninas. Esses tumores são caracterizados por uma apresentação clínica, comportamento biológico e características morfológicas e moleculares variáveis. Dentre os fatores envolvidos em sua patogênese está a exposição ao estrógeno e a ação de seu receptor. O receptor de estrógeno-α (REα) é uma proteína, codificada pelo gene ESR1, amplamente utilizada como marcador molecular de prognóstico para o câncer de mama em mulheres, porém na cadela ainda é pouco utilizado. Neste trabalho, foi avaliada a expressão do gene ESR1 por qPCR em amostras de mama normal, lesões epiteliais não neoplásicas, tumores mamários benignos e malignos. Além disso, fez-se acompanhamento desses animais por um período de 2 anos, avaliando a sobrevida global e a sobrevida livre de doença. Neste estudo foram utilizadas biópsias de 21 cadelas com tumores mamários. No estudo histopatológico foram identificadas 90 lesões, sendo 19 lesões epiteliais não neoplásicas, 2 tumores benignos e 69 tumores malignos. Foi encontrado um maior número de lesões em animais com mais idade (p<0,05). Animais castrados juntamente com a mastectomia apresentaram uma sobrevida maior do que animais inteiros (p<0,05). Observou-se uma menor expressão do gene ESR1 (p < 0,05) nos tumores malignos em relação às mamas normais, lesões epiteliais não neoplásicas e aos tumores benignos. Além disso, verificou-se uma maior expressão em carcinomas de bom prognóstico comparado aos carcinomas do tipo especial (p<0,05). Sendo assim, sugere-se a utilização do gene ESR1 como biomarcador de bom prognóstico em cadelas com lesões mamárias.
Cancer is the most common cause of death in dogs worldwide and mammary tumors is the most common neoplasm in female dogs. These tumors are characterized by variable clinical presentation, biological behavior, morphological and molecular characteristics. Among the factors involved in its pathogenesis is exposure to estrogen and the action of its receptor. The estrogen receptor-α (ERα) is a protein, encoded by the ESR1 gene, widely used as a molecular prognostic marker for breast cancer in women, but it is not used frequently in dogs. In this work, the expression of the ESR1 gene was evaluated by RT-qPCR in samples from normal breast, non-neoplastic epithelial lesions, benign and malignant mammary tumors. Furthermore, these animals were monitored for a period of 2 years, evaluating overall survival and disease-free survival. In this study, biopsies from 21 dogs with mammary tumors were used. In the histopathological study, 90 lesions were identified, 19 non-neoplastic epithelial lesions, 2 benign tumors and 69 malignant tumors. A greater number of lesions were found in older animals (p<0.05). Animals spayed on the same day that mastectomy had a higher survival rate than intact animals. A lower expression of the ESR1 gene (p < 0.05) was observed in malignant tumors compared to normal tissue, non-neoplastic epithelial lesions and benign tumors. Furthermore, there was a higher expression in carcinomas with a good prognosis compared to special types of carcinomas (p<0.05). Therefore, it is suggested to use the ESR1 gene as a biomarker of good prognosis in female dogs with mammary lesions.
Cancer is the most common cause of death in dogs worldwide and mammary tumors is the most common neoplasm in female dogs. These tumors are characterized by variable clinical presentation, biological behavior, morphological and molecular characteristics. Among the factors involved in its pathogenesis is exposure to estrogen and the action of its receptor. The estrogen receptor-α (ERα) is a protein, encoded by the ESR1 gene, widely used as a molecular prognostic marker for breast cancer in women, but it is not used frequently in dogs. In this work, the expression of the ESR1 gene was evaluated by RT-qPCR in samples from normal breast, non-neoplastic epithelial lesions, benign and malignant mammary tumors. Furthermore, these animals were monitored for a period of 2 years, evaluating overall survival and disease-free survival. In this study, biopsies from 21 dogs with mammary tumors were used. In the histopathological study, 90 lesions were identified, 19 non-neoplastic epithelial lesions, 2 benign tumors and 69 malignant tumors. A greater number of lesions were found in older animals (p<0.05). Animals spayed on the same day that mastectomy had a higher survival rate than intact animals. A lower expression of the ESR1 gene (p < 0.05) was observed in malignant tumors compared to normal tissue, non-neoplastic epithelial lesions and benign tumors. Furthermore, there was a higher expression in carcinomas with a good prognosis compared to special types of carcinomas (p<0.05). Therefore, it is suggested to use the ESR1 gene as a biomarker of good prognosis in female dogs with mammary lesions.
Descrição
Palavras-chave
Tumor de mama em cadelas; ESR1; glândula mamária; prognostico; expressão gênica; Canine mammary tumor; mammary gland; prognostic; gene expression
Citação
GÖTZE, Daniela Markus. EXPRESSÃO DO GENE ESR1 EM TUMORES DE MAMA CANINO. 2023. Dissertação (Mestrado) – Curso de Biotecnologia, Universidade do Vale do Taquari - Univates, Lajeado, 19 dez. 2023. Disponível em: http://hdl.handle.net/10737/4325.